Autophagy Regulates Colistin-Induced Apoptosis in PC-12 Cells
نویسندگان
چکیده
منابع مشابه
A Dual Role of P53 in Regulating Colistin-Induced Autophagy in PC-12 Cells
This study aimed to investigate the mechanism of p53 in regulating colistin-induced autophagy in PC-12 cells. Importantly, cells were treated with 125 μg/ml colistin for 12 and 24 h after transfection with p53 siRNA or recombinant plasmid. The hallmarks of autophagy and apoptosis were examined by real-time PCR and western blot, fluorescence/immunofluorescence microscopy, and electron microscopy...
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Laboratory data have demonstrated that cadmium (Cd) may induce neuronal apoptosis. However, little is known about the role of autophagy in neurons. In this study, cell viability decreased in a dose- and time-dependent manner after treatment with Cd in PC-12 cells. As cells were exposed to Cd, the levels of LC3-II proteins became elevated, specific punctate distribution of endogenous LC3-II incr...
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Spinal cord injury (SCI) is one of the most devastating diseases. MicroRNAs (miRNAs) are recognized as key regulators in SCI; however, the role of miR-223 in SCI remains unclear. Herein, our study aimed to explore the effect of miR-223 on lipopolysaccharide (LPS)-induced injury to PC-12 cells. PC-12 cells were treated with different concentrations of LPS, and then cell viability, apoptosis, apo...
متن کاملTrahalose Activates Autophagy and Prevents Hydrogen Peroxide-Induced Apoptosis in the Bone Marrow Stromal Cells
Bone marrow stromal stem cells (BMSCs) play a significant role in cell therapy. These cells quickly die after transplantation to the affected area due to oxidative stress. The natural disaccharide, trehalose which can be known as autophagy inducer. The present study aimed to investigate the role of trehalose in preventing BMSCs from oxidative stress caused by H2O2. BMSCs were isolated from the ...
متن کاملTrahalose Activates Autophagy and Prevents Hydrogen Peroxide-Induced Apoptosis in the Bone Marrow Stromal Cells
Bone marrow stromal stem cells (BMSCs) play a significant role in cell therapy. These cells quickly die after transplantation to the affected area due to oxidative stress. The natural disaccharide, trehalose which can be known as autophagy inducer. The present study aimed to investigate the role of trehalose in preventing BMSCs from oxidative stress caused by H2O2. BMSCs were isolated from the ...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 2015
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.04092-14